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multichannel fluorescence microscopy imager z2  (Carl Zeiss)


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    Structured Review

    Carl Zeiss multichannel fluorescence microscopy imager z2
    Time-dependent <t>fluorescence</t> intensity and representative <t>microscopy</t> images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.
    Multichannel Fluorescence Microscopy Imager Z2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multichannel+fluorescence+microscopy/microscope+z2/pmc12066426-53-34-39
    Average 90 stars, based on 1 article reviews
    multichannel fluorescence microscopy imager z2 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases"

    Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

    Journal: Frontiers in Surgery

    doi: 10.3389/fsurg.2025.1567711

    Time-dependent fluorescence intensity and representative microscopy images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.
    Figure Legend Snippet: Time-dependent fluorescence intensity and representative microscopy images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.

    Techniques Used: Fluorescence, Microscopy, Incubation, Staining

    Comparison of fluorescence intensity between different cell lines after SF stain. (a) Differential fluorescence intensity per cell line. Dotted pattern: glioma cell lines, striped pattern: adenocarcinoma cell lines. Error bars indicate the 95% confidence interval. *indicates a significance level of 0.05. For cell lines Ln18, T98G, and Ln229 differences to all of the adenocarcinoma were significant at the 0.05 level. (b–i) Representative fluorescence microscopy images of the stained cell lines: (b) Ln18, (c) T98G, (d) Ln229, (e) U87MG, (f) MCF7, (g) SW48, (h) MDA, and (i) OV-MZ-6. Scale bars: 25 µm.
    Figure Legend Snippet: Comparison of fluorescence intensity between different cell lines after SF stain. (a) Differential fluorescence intensity per cell line. Dotted pattern: glioma cell lines, striped pattern: adenocarcinoma cell lines. Error bars indicate the 95% confidence interval. *indicates a significance level of 0.05. For cell lines Ln18, T98G, and Ln229 differences to all of the adenocarcinoma were significant at the 0.05 level. (b–i) Representative fluorescence microscopy images of the stained cell lines: (b) Ln18, (c) T98G, (d) Ln229, (e) U87MG, (f) MCF7, (g) SW48, (h) MDA, and (i) OV-MZ-6. Scale bars: 25 µm.

    Techniques Used: Comparison, Fluorescence, Staining, Microscopy

    (A) resected metastasis after intravenous application of SF in conventional fluorescence microscopy. Scale bar: 50 µm. (B) H&E staining of the same metastasis as a comparison. Scale bar: 50 µm.
    Figure Legend Snippet: (A) resected metastasis after intravenous application of SF in conventional fluorescence microscopy. Scale bar: 50 µm. (B) H&E staining of the same metastasis as a comparison. Scale bar: 50 µm.

    Techniques Used: Fluorescence, Microscopy, Staining, Comparison

    Related Articles

    Fluorescence:

    Article Title: Differential expression of tension-sensitive HOX genes in fibroblasts is associated with different scar types
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).

    Article Title: Tension-sensitive HOX gene expression in fibroblasts for differential scar formation.
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3 min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1 μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model
    Article Snippet: The secondary antibody for K16 was AF488-conjugated donkey anti-mouse IgG (ThermoFisher); for hBD2 AF546-conjugated donkey anti-goat (ThermoFisher), and for CD3 AF647-conjugated donkey anti-rabbit (ThermoFisher). .. Sections were photographed using a microscope (AxioImager M2; Zeiss, Sliedrecht, the Netherlands) and a high resolution color camera for bright field microscopy (AxioCam 105 color, Zeiss) and a high resolution b/w camera for multichannel fluorescence microscopy (AxioCam 503 Mono). .. Mean epidermal thickness was calculated using AxioVision software version 4.8 (Zeiss) or ZEN blue edition version 2.3 (Zeiss) as epidermal area divided by epidermal surface length.

    Microscopy:

    Article Title: Differential expression of tension-sensitive HOX genes in fibroblasts is associated with different scar types
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).

    Article Title: Tension-sensitive HOX gene expression in fibroblasts for differential scar formation.
    Article Snippet: To label nuclei, 4’,6-diamidino-2-phenylindole (DAPI, 1:50000 dilution, Molecular Probes) was treated in the cells for 3 min. .. Cells were then imaged using multichannel fluorescence microscopy (Carl Zeiss). .. Cells were cultured on polydimethylsiloxane (PDMS) (Dow Corning, USA), which were pretreated with oxygen plasma (Convance, Femto Science, Korea) for 1 min at a pressure of 0.7 Torr followed by 1 μg/cm2 of fibronectin (Invitrogen, USA) coating for 120 min at room temperature.

    Article Title: Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model
    Article Snippet: The secondary antibody for K16 was AF488-conjugated donkey anti-mouse IgG (ThermoFisher); for hBD2 AF546-conjugated donkey anti-goat (ThermoFisher), and for CD3 AF647-conjugated donkey anti-rabbit (ThermoFisher). .. Sections were photographed using a microscope (AxioImager M2; Zeiss, Sliedrecht, the Netherlands) and a high resolution color camera for bright field microscopy (AxioCam 105 color, Zeiss) and a high resolution b/w camera for multichannel fluorescence microscopy (AxioCam 503 Mono). .. Mean epidermal thickness was calculated using AxioVision software version 4.8 (Zeiss) or ZEN blue edition version 2.3 (Zeiss) as epidermal area divided by epidermal surface length.

    Immunofluorescence:

    Article Title: Physicochemically Tuned Myofibroblasts for Wound Healing Strategy
    Article Snippet: During the staining step, we treat α-smooth muscle actin (α-SMA) antibody (Abcam) (with 1:100 dilution) for 12 hours, Alex Flour 488-fluorescence antibody (Invitrogen) (with 1:200 dilution) for 6 hours, Alex Fluor 568-phalloidin (Invitrogen) (with 1:50 dilution) for 20 min, and 4’,6-diamidino-2-phenylindole (DAPI, Molecular Probe) (with 1:50000 dilution) for 3 min. .. The multichannel fluorescence microscopy (Zeiss) was used for taking immunofluorescence images. ..

    Staining:

    Article Title: Fibrous Matrix Architecture-Dependent Activation of Fibroblasts with a Cancer-Associated Fibroblast-like Phenotype.
    Article Snippet: Cancer-associated fibroblasts (CAFs) are one of the most prevalent cell types within the tumor microenvironment (TME).. While several physicochemical cues from the TME, including growth factors, cytokines, and ECM specificity, have been identified as essential factors for CAF activation, the precise mechanism of how the ECM architecture regulates CAF initiation remains elusive.. Using a gelatin-based electrospun fiber mesh, we examined the effect of matrix fiber density on CAF activation induced by MCF-7 conditioned media (CM).



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    Time-dependent <t>fluorescence</t> intensity and representative <t>microscopy</t> images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.
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    Image Search Results


    Time-dependent fluorescence intensity and representative microscopy images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.

    Journal: Frontiers in Surgery

    Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

    doi: 10.3389/fsurg.2025.1567711

    Figure Lengend Snippet: Time-dependent fluorescence intensity and representative microscopy images. (a) Mean fluorescence intensity in Ln229 cells as a function of incubation time. Error bars indicate the 95% confidence interval. (b–g) Fluorescent images of a Ln229 cell monolayer stained with SF (b,d) , PI (c,f) and Hoechst (d,g) for 40 min. Exposure time was adjusted for each picture individually. Dynamic display range was set to the minimum/maximum non-zero value for the green and DAPI channels; for the red channel the maximum pixel value was set to the camera's maximum pixel value of 16,384. White bar ∼50 μm. (a–d) Incubation of living cells, (e,f) Incubation of fixed cells.

    Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

    Techniques: Fluorescence, Microscopy, Incubation, Staining

    Comparison of fluorescence intensity between different cell lines after SF stain. (a) Differential fluorescence intensity per cell line. Dotted pattern: glioma cell lines, striped pattern: adenocarcinoma cell lines. Error bars indicate the 95% confidence interval. *indicates a significance level of 0.05. For cell lines Ln18, T98G, and Ln229 differences to all of the adenocarcinoma were significant at the 0.05 level. (b–i) Representative fluorescence microscopy images of the stained cell lines: (b) Ln18, (c) T98G, (d) Ln229, (e) U87MG, (f) MCF7, (g) SW48, (h) MDA, and (i) OV-MZ-6. Scale bars: 25 µm.

    Journal: Frontiers in Surgery

    Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

    doi: 10.3389/fsurg.2025.1567711

    Figure Lengend Snippet: Comparison of fluorescence intensity between different cell lines after SF stain. (a) Differential fluorescence intensity per cell line. Dotted pattern: glioma cell lines, striped pattern: adenocarcinoma cell lines. Error bars indicate the 95% confidence interval. *indicates a significance level of 0.05. For cell lines Ln18, T98G, and Ln229 differences to all of the adenocarcinoma were significant at the 0.05 level. (b–i) Representative fluorescence microscopy images of the stained cell lines: (b) Ln18, (c) T98G, (d) Ln229, (e) U87MG, (f) MCF7, (g) SW48, (h) MDA, and (i) OV-MZ-6. Scale bars: 25 µm.

    Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

    Techniques: Comparison, Fluorescence, Staining, Microscopy

    (A) resected metastasis after intravenous application of SF in conventional fluorescence microscopy. Scale bar: 50 µm. (B) H&E staining of the same metastasis as a comparison. Scale bar: 50 µm.

    Journal: Frontiers in Surgery

    Article Title: Fluorescein-distribution in confocal laser endomicroscopy allows for discrimination between primary brain tumours and metastases

    doi: 10.3389/fsurg.2025.1567711

    Figure Lengend Snippet: (A) resected metastasis after intravenous application of SF in conventional fluorescence microscopy. Scale bar: 50 µm. (B) H&E staining of the same metastasis as a comparison. Scale bar: 50 µm.

    Article Snippet: These specimens were snap frozen, sliced on a cryostat, stained with conventional Hematoxylin & Eosin (H&E) and on the corresponding following slide, conventional fluorescence microscopy was performed to detect the SF fluorescence with a multichannel fluorescence microscopy (Imager Z2, Carl Zeiss Meditec AG).

    Techniques: Fluorescence, Microscopy, Staining, Comparison